Expression profiling of microRNA using oligo DNA arrays
نویسندگان
چکیده
منابع مشابه
Profiling microRNA expression using sensitive cDNA probes and filter arrays.
MicroRNAs (miRNAs) are small noncoding RNAs (approximately 22 nucleotides) that have recently emerged as important regulators of gene expression in both plants and animals. With few exceptions, however, the target genes and the expression levels of most miRNAs are unknown. Here we show that direct random-primed cDNA synthesis on either chemically synthesized small RNAs (21-22 nucleotides) or ge...
متن کاملSystematic enrichment analysis of microRNA expression profiling studies in endometriosis
Objective(s): The purpose of this study was to conduct a meta-analysis on human microRNAs (miRNAs) expression data of endometriosis tissue profiles versus those of normal controls and to identify novel putative diagnostic markers. Materials andMethods: PubMed, Embase, Web of Science, Ovid Medline were used to search for endometriosis miRNA expression profiling studies of endometriosis. The miRN...
متن کاملExpression profiling: DNA arrays in many guises.
DNA arrays have become the preferred method for large-scale expression measurement. Such data are needed in view of the large amounts of sequence data available: expression levels in a number of different tissues or situations provide a first step toward functional characterisation of new entities revealed by DNA sequencing. Although the basic principle of measurement is in all cases based on h...
متن کاملMicroRNA expression profiling on arrays enhanced with locked nucleic acids
Here we describe a new array system (miRCURYTM LNA Array) for global microRNA (miRNA) profiling of biological samples. The miRCURY LNA Array system uses locked nucleic acid (LNATM), a high-affinity nucleotide analog, to enhance the array capture probes, giving them high sensitivity and excellent selectivity for miRNA targets. We have simultaneously developed a straightforward method for labelin...
متن کاملsystematic enrichment analysis of microrna expression profiling studies in endometriosis
objective(s): the purpose of this study was to conduct a meta-analysis on human micrornas (mirnas) expression data of endometriosis tissue profiles versus those of normal controls and to identify novel putative diagnostic markers. materials andmethods: pubmed, embase, web of science, ovid medline were used to search for endometriosis mirna expression profiling studies of endometriosis. the mirn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Methods
سال: 2008
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2007.10.010